Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 15;15(3):952. doi: 10.3390/pharmaceutics15030952

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

New NF55 analogs form nanoparticles which led to high reporter levels post-transfection both in vitro and in vivo. Complex formation between CPP and (a) mRNA or (b) pDNA assessed by the accessibility of nucleic acid to fluorescent nucleic acid binding dye after the addition of CPPs. CPP/NA complexes formed at charge ratios (CR) 1:1, 2:1, and 3:1 with CPPs in excess. Free nucleic acid at the same concentration was used as the controls (100%). The stability of pre-formed (c) CPP/mRNA and (d) CPP/pDNA nanoparticles to enzymatic digestion by proteinase K. The stability of pre-formed (e) CPP/mRNA and (f) CPP/pDNA nanoparticles to heparin displacement.