Table 1:
Native mass spectrometry of coronavirus polymerase complexes.
| Sample | Major Peak(s) (Da) | Minor Peak(s) (Da) |
|---|---|---|
| PEDV nsp12+8+7 | 185,120 ± 70 | 109,450 ± 40 |
| PEDV A382R- nsp12+8+7 | 109,533 ± 60 185,120 ± 30 |
218,880 ± 50 |
| PEDV V848R- nsp12+8+7 | 185,200 ± 200 | 370,700 ± 600 109,630 ± 200 |
| PEDV nsp12+8+8L7 | 184, 670 ± 60 | 109,420 ± 30 |
| SARS-CoV-2 nsp12+8+7 | 186,240 ± 70 | 110,680 ± 20 |
| SARS-CoV-2 L387R- nsp12+8+7 | 186,400 ± 100 | 110,900 ± 100 22,400 ± 100 |
| SARS-CoV-2 T853R- nsp12+8+7 | 186,190 ± 50 | 22,300 ± 50 |
| SARS-CoV-2 nsp12+8+8L7 | 186,270 ± 40 | 110,600 ± 40 |
Major and minor species from native mass spectrometry experiments are listed for each complex tested with their respective (±) standard deviations. The expected protein or complex associated with each weight are as follows: ~22 kDa – nsp8, ~110 kDa – nsp12, ~185 kDa – intact core complex, 218 kDa – nsp12 dimer, 370 kDa – complex dimer (See also Table S2 and Figure S4).