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[Preprint]. 2023 Mar 19:2023.03.16.532975. [Version 1] doi: 10.1101/2023.03.16.532975

Extended Data Fig. 14: Curved adhesions, but not focal adhesions, are prevalent in 3D ECMs.

Extended Data Fig. 14:

a, x-y view (Z0 plane) and x-z view (Y0 plane) of Z-stack images of immunolabeled ITGβ5 in U2OS cells expressing FCHo2-GFP. The cells are embedded in 3D ECM made of vitronectin fibres labelled with AF647-collagen. Zoom-in x-y images of the yellow-box area show abundant curved adhesions indicated by the colocalization of FCHo2 and ITGβ5. Curved adhesions form along vitronectin fibres at the middle (Z0 plane), bottom (Z1 plane), and top (Z2 plane) of cells. The zoom-in of the x-y slide at the Z0 plane is also shown in Fig. 5g (top). b, x-y view (Z0 plane) and x-z view (Y0 plane) of Z-stack images of immunolabeled vinculin and ITGβ5 in U2OS. The cells are embedded in 3D ECM made of vitronectin fibres labelled with AF647-collagen. Zoom-in x-y images of the yellow-box area do not show clear focal adhesions indicated by the colocalization of vinculin and ITGβ5. Examination of different imaging planes, the middle (Z0 plane), bottom (Z1 plane), and top (Z2 plane) of cells show that the colocalization of vinculin and ITGβ5 is sparse. A zoom-in of the x-y view (Z0 plane) is also shown in Fig. 5g (bottom). Scale bars: 10 μm.