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[Preprint]. 2023 Mar 14:2023.03.14.532634. [Version 1] doi: 10.1101/2023.03.14.532634

Figure 6: Differential regulation of kinases by biased ligands and phosphodeficient receptors.

Figure 6:

Biased phosphorylation of various kinases identified from the global phosphoproteomics data including (A) ERK1, (B) RAF1, (C) BRAF, (D) Casein kinase 2 (CSNK2A3/CSNK2A1), (E) Src family of protein tyrosine kinases (FYN/YES1/LCK/SRC), and (F) JNKs (JNK1/JNK3). Data is normalized to vehicle treatment and n=2 technical replicates of six pooled biological replicates. Mean ± SEM. *P<.05 by one-way ANOVA, Tukey’s post hoc testing. (G) Representative western blot of phosphorylated ERK1/2 (pERK 1/2) and total ERK1/2 (tERK 1/2) following stimulation with vehicle control or 100 nM of CXCL11 for five minutes. (H-J) Quantification of western blots of pERK1/2 levels at 5, 30, and 60 minutes. Mean ± SEM, n=4. *P<.05 by two-way ANOVA, Dunnet’s post hoc testing denotes comparisons between a specific ligand/receptor combination to the same ligand at CXCR3-WT. See S8 for quantification of western blots grouped by receptor.