KEY RESOURCES TABLE
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Donkey polyclonal anti-rabbit IgG peroxidase conjugated | Rockland | Cat#611–7302; RRID:AB_219747 |
| Sheep polyclonal anti-mouse IgG peroxidase conjugated | Rockland | Cat#610–603-002; RRID:AB_219694 |
| Mouse monoclonal anti-phospho-p44/42 MAPK 1/2 (ERK1/2) (Thr202/Tyr204) | Cell Signaling Technologies | Cat#9106; RRID:AB_331768 |
| Rabbit polyclonal anti-MAPK 1/2 (ERK1/2) | Millipore Sigma | Cat#06–182; RRID:AB_310068 |
| Mouse monoclonal anti-human CD183 (CXCR3) PE conjugated | R&D Systems | Cat#FAB160P; RRID:AB_2086755 |
| Mouse monoclonal anti-human CD183 (CXCR3) APC conjugated | BioLegend | Cat#353707; RRID:AB_10962949 |
| Bacterial Strains | ||
| XL10-Gold Ultracompetent E. Coli | Agilent | Cat#200315 |
| Chemicals, peptides, and recombinant proteins | ||
| Recombinant Human CXCL9 | Peprotech | Cat#300–26 |
| Recombinant Human CXCL10 | Peprotech | Cat#300–12 |
| Recombinant Human CXCL11 | Peprotech | Cat#300–46 |
| GlutaMax | Gibco | Cat#35050061 |
| Antibiotic-Antimycotic | Gibco | Cat#15240062 |
| Fugene 6 | Promega | Cat#E2691 |
| Lipofectamine 2000 | Invitrogen | Cat#11668019 |
| para-Nitrophenyl Phosphate | Sigma-Aldrich | Cat#4876 |
| 2,3-dimercapto-1-propanol | Sigma-Aldrich | Cat#64046 |
| FlAsH-EDT2 | Santa Cruz Biotechnology | Cat#sc-363644 |
| Coelenterazine h | Cayman Chemical | Cat#16894 |
| Coelenterazine h | NanoLight Technology | Cat#301 |
| Coelenterazine 400a | Cayman Chemical | Cat#16157 |
| PhosSTOP | Sigma-Aldrich | Cat#4906845001 |
| cOmplete Protease Inhibitor Cocktail | Sigma-Aldrich | Cat#11697498001 |
| SuperSignal West Pico PLUS Chemiluminescent Substrate | Thermo Fischer Scientific | Cat#34580 |
| Lenti-X Concentrator | Takara Bio | Cat#631232 |
| Polybrene | Sigma-Aldrich | Cat#TR-1003 |
| Critical commercial assays | ||
| qPCR Lentivirus Titration Kit | Applied Biological Materials (ABM) | Cat#LV900 |
| QuikChange Lightning SiteDirected Mutagenesis Kit | Agilent | Cat#210518 |
| ChemoTx 5 μm Chemotaxis System | Neuroprobe | Cat#116–5 |
| Mass Spectrometry Resources | ||
| Ni-NTA Superflow Agarose Beads | Qiagen | Cat#30410 |
| BCA Protein Assay Kit | ThermoFisher Scientific | Cat#A53225 |
| TMT-11 reagent kit | ThermoFisher Scientific | Cat#A34808 |
| Trypsin | Promega | Cat#V5117 |
| Empore Octadecyl C18, 47 mm | Supleco | Cat#66883-U |
| Waters tC18 SepPak | Waters | Cat#WAT054925 |
| Deposited Data | ||
| Mass Spectrometry Proteomics Data | JPOST | JPST001599 (Accession Key 6844) |
| Molecular Dynamics Simulations | GPCRmd | 1485 |
| Experimental Models: Cell Lines | ||
| Human: 293 | ATCC | Cat#CRL-1573; RRID:CVCL_0045 |
| Human: 293 | ATCC | Cat#CRL-3216; RRID:CVCL_0063 |
| Human: 293 β-arrestin 1/2 Knock Out | Asuka Inoue | (Alvarez-Curto et al., 2016) |
| Human: 293 GRK 2, 3, 5, 6 Knock Out | Asuka Inoue | (Pandey et al., 2021b) |
| Human: Jurkat, Clone E6–1 | ATCC | Cat#TIB-152; RRID:CVCL_0367 |
| Recombinant DNA | ||
| CXCR3 | Rajagopal Lab | N/A |
| CXCR3-S355A/S356A | This work | N/A |
| CXCR3-T360A/S361A/ | This work | N/A |
| CXCR3-T360A/S361A/S364A/S366A | Rajagopal Lab (Smith et al., 2021) | N/A |
| CXCR3-L344X | Rajagopal Lab (Smith et al., 2017) | N/A |
| Gαi1-RLuc8 | Bryan Roth Lab (Olsen et al., 2020) | N/A |
| Gγ9-GFP2 | Bryan Roth Lab (Olsen et al., 2020) | N/A |
| Gβ3 | Bryan Roth Lab (Olsen et al., 2020) | N/A |
| CXCR3-RlucII | This work | N/A |
| CXCR3- S355A/S356A -RlucII | This work | N/A |
| CXCR3-T360A/S361A/-RlucII | This work | N/A |
| CXCR3-T360A/S361A/S364A/S366A-RlucII | This work | N/A |
| CXCR3-L344X-RlucII | This work | N/A |
| βarr2-mKO | Rajagopal Lab (Smith et al., 2021) | N/A |
| Myrpalm-mVenus | Rajagopal Lab (Smith et al., 2017) | N/A |
| 2x-Fyve-mvenus | Rajagopal Lab (Smith et al., 2017) | N/A |
| CXCR3-GFP | Rajagopal Lab (Smith et al., 2017) | N/A |
| CXCR3- S355A/S356A -GFP | This work | N/A |
| CXCR3-T360A/S361A/-GFP | This work | N/A |
| CXCR3-T360A/S361A/S364A/S366A-GFP | This work | N/A |
| CXCR3-L344X-GFP | This work | N/A |
| βarr2-RFP | Marc Caron Lab | N/A |
| βarr2-FlAsH biosensors 1–6 | Louis Luttrell Lab (Lee et al., 2016) | N/A |
| CXCR3-SmBiT | This work | N/A |
| CXCR3- S355A/S356A -SmBiT | This work | N/A |
| CXCR3-T360A/S361A/-SmBiT | This work | N/A |
| CXCR3-T360A/S361A/S364A/S366A-SmBiT | This work | N/A |
| CXCR3-L344X-SmBiT | This work | N/A |
| GRK2-LgBiT | Asuka Inoue Lab | N/A |
| GRK3-LgBiT | Asuka Inoue Lab | N/A |
| GRK5-LgBiT | Asuka Inoue Lab | N/A |
| GRK6-LgBiT | Asuka Inoue Lab | N/A |
| pLenti-CXCR3 | This work | N/A |
| pLenti-CXCR3-S355A/S356A | This work | N/A |
| pLenti-CXCR3-T360A/S361A | This work | N/A |
| pLenti-CXCR3-T360A/S361A/S364A/S366A | This work | N/A |
| pLenti-CXCR3- L344X | This work | N/A |
| pMD2.G | Addgene | Cat#12259; RRID:Addgene_12259 |
| psPAX2 | Addgene | Cat#12260; RRID:Addgene_12260 |
| PX459 | Addgene | Cat#62988; RRID:Addgene_62988 |
| Software and algorithms | ||
| GraphPad Prism | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ | (Schneider et al., 2012) | https://imagej.nih.gov/ij/ |
| Adobe Illustrator | Adobe | https://www.adobe.com/ |
| Excel | Microsoft | https://www.microsoft.com/en-us/microsoft-365/excel |
| Database for Annotation, Visualization, and Integrated Discovery (DAVID) | (Huang da et al., 2009a; b) | https://david.ncifcrf.gov/home.jsp |
| Kinase Enrichment Analysis | (Lachmann and Ma’ayan, 2009) | https://www.maayanlab.net/KEA2/ |
| Modification Motifs | (Bailey et al., 2006; Cheng et al., 2019) | https://meme-suite.org/meme/tools/momo |
| GProX | (Rigbolt et al., 2011) | http://gprox.sourceforge.net/ |
| MaxQuant | (Tyanova et al., 2016a) | https://www.maxquant.org/ |
| FlowJo | Becton, Dickinson & Company | https://www.flowjo.com/ |
| ImageLab | Bio-Rad | https://www.bio-rad.com/en-us/product/image-lab-software |
| BioRender | BioRender | https://biorender.com/ |
| GPCRdb | (Kooistra et al., 2021) | https://gpcrdb.org/ |