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[Preprint]. 2023 Mar 20:2023.03.19.533379. [Version 1] doi: 10.1101/2023.03.19.533379

Fig. 5. Interference with host endocytic and retromer-mediated trafficking pathways allows partial rescue of vacuole integrity defect.

Fig. 5.

(A) Effect of si-Rab5B on vacuole integrity. Left: immunoblot of SDS-polyacrylamide gel probed with anti-Rab5B after depletion with si-Rab5B or control scrambled si-RNA. Right: the percentage of cytosolic bacteria from BMDMs treated with si-Control or si-Rab5B, and challenged with noted L. pneumophila strains for 2 hrs, followed by probing as described (Materials and Methods). (B) Effect of si-SNX1 on vacuole integrity. Left: immunoblot of SDS-polyacrylamide gel probed with anti-SNX1 after depletion with si-SNX1 or control scrambled si-RNA. Right: the percentage of cytosolic bacteria from BMDMs treated with si-Control or si-SNX1 and challenged with noted L. pneumophila strains for 2 hrs, followed by probing as described (Materials and Methods). For immunoblotting, β-actin was used as loading control. The data shown are mean ± SEM, using three biological replicates, with at least 100 LCVs counted per biological replicate. Statistical analysis was conducted using unpaired two-tailed Student’s t test and significance displayed as **p < 0.01 or ****p < 0.0001.