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[Preprint]. 2023 Mar 23:2023.03.18.533297. [Version 1] doi: 10.1101/2023.03.18.533297

Figure 1 -. Gata3+Foxp3+Tregs are a previously unappreciated feature of islet autoimmunity.

Figure 1 -

A: Representative flow cytometry for Gata3, T-bet and Rorγt expression gated on Foxp3+CD4+ T cells (left), and frequency of Gata3+ cells among Foxp3+CD4+ T cells in islets and pancreatic lymph nodes (pLN) of 10–12 week-old female NOD mice (right). The frequency of Rorγt+ cells is shown for comparison. (n=4–11/group) B: Representative flow cytometry for ST2 expression on the surface of Foxp3+CD4+ T cells in islets, pLN and spleen (left). Summary data are shown for ST2 expression in islet and pLN Tregs in 10- and 16-week-old female NOD mice (n=5/group). C: Quantitative PCR measurement for Il-33 and Gata3 mRNA in islets from female NOD mice at the indicated age (n=7–8/group). D: Western blot measurement of IL-33 protein expression in islets from female NOD (n=2/age group) and NOD.scid (n=3) mice. ns P>0.05; *P<0.05; ** P<0.01; ****P<0.0001 (A: Mann-Whitney test, B,C: One-way ANOVA)