Extended Data Fig. 9 ∣. Exploratory analyses of single-cell-type pseudobulk data from the dlPFC.

UMAP plots of pseudobulk libraries reveal that the latent structure is driven primarily by (a) cell type and not (b) age. (c) Single-nucleus libraries were aggregated across all cell types to approximate bulk tissue RNA sequencing libraries. Age effects from pseudobulk dlPFC libraries were then compared to age effects from bulk dlPFC libraries, showing a strong positive correlation. Colors denote whether age effects were considered significant for both datasets (EMMA test uncorrected P < 0.05). Error bands represent the 95% confidence interval of linear model predictions. (d) Similarly, age prediction on pseudobulk libraries using the wbaDEGs model (see Methods) developed from analysis of bulk RNA-seq data shows a similar positive correlation (linear model statistics are presented) and accuracy to predictions on bulk tissue libraries. Error bands represent the 95% confidence interval of linear model predictions.