Extended Data Fig. 1 ∣. Sample and study design.
(a) Location of Cayo Santiago off the southeastern coast of Puerto Rico. (b) Age distribution of individuals sampled for the bulk tissue RNA sequencing dataset. Selected individuals span the natural adult age distribution and are evenly balanced between sexes. (c) Age distribution of individuals per brain region (excludes samples not passing laboratory or bioinformatic quality control). (d) Age distribution of females sampled for the single-nucleus RNA sequencing dataset. Selected individuals span the natural age distribution and are evenly balanced between two naturally occurring social groups (HH and KK) on Cayo Santiago. (e) Hazard rates from a previously published demographic model of Cayo Santiago macaques (ref. 2) demonstrate that individuals in the age range of our sample experience age-associated increases in mortality risk (N = 11,659 biologically independent animals). Error bars represent the mean hazard rate ± the standard error.