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[Preprint]. 2023 Mar 23:2023.03.23.533943. [Version 1] doi: 10.1101/2023.03.23.533943

Fig. 4. Trifluridine and adefovir dipivoxil suppress VACV DNA replication and post-replicative gene expression, but not early gene expression.

Fig. 4.

(A-C) HFFs were infected with vEGluc (A), vIntGluc (B), and vLGluc (C) at an MOI of 2 and treated with indicated compounds at 10 μM, respectively, or vehicle DMSO. Gluc activities were measured at 4 h (vEGluc), 8 h (vIntGluc), and 8 h (vLGluc), respectively. (D) HFFs were infected with VACV at an MOI of 2 in the presence of indicated compounds at 10 μM for 8 h. relative amounts of Viral DNA were determined by real-time PCR using VACV specific primers. At least three repeats were performed for all assays. *0.01<p ≤ 0.05; ***0.001<p ≤ 0.01; ns, not significant.