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[Preprint]. 2023 Mar 26:2023.03.25.534233. [Version 1] doi: 10.1101/2023.03.25.534233
Fetal Bovine Serum Gemini Bio-Products Cat#100-500
B-27 Supplement (50x) Fisher Scientific Cat#A3582801
N-2 Supplement (100x) Thermo Fisher Scientific Cat#17502048
GlutaMAX Supplement Thermo Fisher Scientific Cat#35050061
Neurobasal Medium Thermo Fisher Scientific Cat#21103049
Hanks’ Balance Salt Solution (HBSS), calcium, magnesium, no phenol red Thermo Fisher Scientific Cat#14-025-076
Penicillin/Streptomycin Thermo Fischer Scientific Cat#15140-122
Papain Worthington Cat#LK003178
DNase I Sigma-Aldrich Cat#D5025
Poly-D-Lysine Thermo Fischer Scientific Cat#A3890401
Fast Green FCF Sigma-Aldrich Cat#F7252
32% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat#15614-S
Fluoromount-G Southern Biotech Cat#0100-01
Glutaraldehyde 25% Aqueous Solution Electron Microscopy Sciences Cat#16220
TTX Hello Bio Cat#HB1035
AP5 Hello Bio Cat#HB0225
NBQX Hello Bio Cat#HB0442
STO-609 Sigma-Aldrich Cat#S1318
Compound-991 Selleck Chem Cat#S8654
DMSO Santa Cruz Biotechnology Cat#sc-358801
Mini-Protean TGX (4-20%) SDS-PAGE BioRad Cat#4561093
Trans-Blot Turbo Nitrocellulose Transfer Packs, Mini, 0.2 μm BioRad Cat#1704158
PVDF Membrane Immobilon Cat#IPL0010
Intercept Blocking Buffer LI-COR Biosciences Cat#927-60003
N-PER Neuronal Protein Extraction Reagent Thermo Fisher Scientific Cat#87792
Phosphotase Inhibitor Sigma-Aldrich Cat#P5726
Phosphotase Inhibitor Cocktail 3 Sigma-Aldrich Cat#P0044
COmplete Protease Inhibitor Sigma-Aldrich Cat#11836170001
Benzonase EMD Millipore Cat#70664-3
Laemmli Buffer (4x) BioRad Cat#1610747
anti-AMPKα Cell Signaling Cat#2532S
2-Mercaptoethanol BioRad Cat#161-0710
anti-phospho-AMPKα Cell Signaling Cat#2535S
anti-MFF Protein Tech Cat#17090-1-AP
anti-phospho-MFF Affinity Biosciences Cat#AF2365
anti-MTFR1L Atlas Antibodies #HPA027124
anti-phospho-MTFR1L Tilokani et al, 2022 N/A
anti-Lphn3 R&D Systems MAB5916
anti-Gapdh Cell Signaling Technologies 2118
Revert 700 Total protein stain LI-COR 926-11010
pCAG-TdTomato Lewis et al, 2016 N/A
pCAG-mtDsRed Courchet et al 2013 N/A
pCAG-mTAGBFP2 Lewis et al. 2018 N/A
pCAG-mtYFP Lewis et al. 2016 N/A
pCAG-mtmTAGBFP2 Lewis et al. 2016 N/A
pCAG-Kir2.1 t2a TdTomato Addgene plasmid #60598
pCMV6-Lphn3 Origene MR212027
pGFP-C-shLenti scrambled negative control Origene TR30021
pGFP-C-shLenti Lphn3 shRNA-D Origene Custom
ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/
GraphPad PRISM GraphPad Software Inc. https://www.graphpad.com/scientific-software/prism/
Trans-Blot Turbo BioRad
Vibratome - Leica VT1200 Leica
Picospritzer III
ECM 830 Electro Square Porator BTX
A1R Confocal Microscope with NISElements Nikon https://www.microscope.healthcare.nikon.com/products/confocal-microscopes/a1hd25-a1rhd25