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[Preprint]. 2023 Mar 24:2023.03.22.533831. [Version 1] doi: 10.1101/2023.03.22.533831

Figure 5. Genipin rescues sensory neuron degeneration in FD.

Figure 5.

A) Schematic of survival assay. B) Healthy or FD cells were treated with genipin from day 12 on (when neurons are born) and monitored for survival for 21 days. Cells were fixed on the indicated days and stained for BRN3A, TUJ1 and DAPI. Arrows indicate healthy, ganglia-like SN clusters. C) Image quantification of B. n=8, Two-way ANOVA followed by Šídák multiple comparisons. **p<0.005, ***p<0.001, ****p<0.0001. D,E, Genipin increases neurite length and number in FD cells. D) Representative images of neurite length. E) Measurement of neurite length (number of pixels) of D. n=6–8 biological replicates, one-way ANOVA followed by Tukey’s multiple comparisons. ns, non-significant, ****p<0.0001. All graphs show mean ± s.d. Data from iPSC-FD-S2 and iPSC-FD-S3 are pooled as FD in C, and E.