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[Preprint]. 2023 Mar 23:2023.03.19.533334. [Version 1] doi: 10.1101/2023.03.19.533334

Figure 4. iPSCs EVs resolve inflammation by transitioning macrophages to an “M2” phenotype and reduce ROS levels.

Figure 4.

(A) In a “post-treat” regime, where RAW264.7s were stimulated with LPS, treated with EVs before lysis, anti-inflammatory macrophage markers/cytokine mRNA expression levels were quantified via RT-qPCR (n=3). (B) RAW264.7 mouse macrophages were pre-treated with EVs before LPS stimulation (100 ng/mL) and subsequent ROS quantification using a H2DCFDA fluorescent dye along with fluorescence quantification via plate reader (n=6). All values were expressed as mean ± standard deviation (*p < 0.05, **p < 0.01, ***p < 0.001), ****p < 0.0001).