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[Preprint]. 2023 Mar 23:2023.03.19.533334. [Version 1] doi: 10.1101/2023.03.19.533334

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Figure 5. — (A) HUVECs were pre-treated with EVs for 24 hours at a dose of 5E9 particles/mL before stimulation with 10 ng/mL TNF for 16 hours before lysing and quantification the endothelial adhesion marker, VCAM1 via RT-qPCR. (B) We looked to confirm the anti-inflammatory effects of our EV samples in a human LPS-stimulated THP1 macrophage assay. The conditioned media of stimulated THP1s was collected and TNF- levels were quantified via ELISA. All values were expressed as mean ± standard deviation (*p < 0.05, ****p < 0.0001).