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[Preprint]. 2023 Mar 20:rs.3.rs-2405644. [Version 1] doi: 10.21203/rs.3.rs-2405644/v1

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Extended Data Figure 6. — a. Schematic representation of the generation of MALAT1-null HEK293T cells by using a double excision CRISPR-knockout method. The MALAT1-knockout cells were verified by DNA sequencing as lacking the fragment between the two sgRNA sites.

b. qPCR of MALAT1 in control and MALAT1-knockout HEK293T cells.

c. qPCR of Malat1 in MALAT1-knockout HEK293T cells with or without piggyBac-mediated expression of Malat1.