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[Preprint]. 2023 Mar 20:rs.3.rs-2405644. [Version 1] doi: 10.21203/rs.3.rs-2405644/v1

Extended Data Figure 7. Effect of Tead3 knockdown on RANKL-induced osteoclastogenesis and expression of Nfatc1 and Ctsk.

Extended Data Figure 7.

a. Immunoblotting of Tead3 and Hsp90 in RAW264.7 cells transfected with two independent Tead siRNAs or scrambled negative control (NC).

b. TRAP staining images (left panel) and quantification (right panel) of control and Tead3-knockdown RAW264.7 cells treated with RANKL (50 ng/mL) for 5 days. Multinucleated TRAP-positive cells (outlined by dashed lines) were counted. Scale bars: 100 μm. Statistical significance was determined by a two-tailed unpaired t-test. Error bars are s.e.m.

c. Immunoblotting of Nfatc1, Ctsk, and β-actin in control and Tead3-knockdown RAW264.7 cells treated with RANKL for 3 days and 5 days.

d. Immunoblotting of Tead3 and Hsp90 in control and Malat1-knockdown RAW264.7 cells transfected with Tead3 siRNA or scrambled negative control (NC).