Extended Data Figure 2. Malat1 is a negative regulator of osteoporosis and bone metastasis.

a-f. μCT-based measurements of the trabecular bone volume per tissue volume (BV/TV, a and b), trabecular number (Tb.N, c and d), and trabecular thickness (Tb.th, e and f) in the femurs from 6-month-old male (n = 5-7 mice per group) and female (n = 7-10 mice per group) Malat1^+/+, Malat1^−/−, and Malat1^−/−;Malat1^Tg/Tg mice, with left and right femurs for each mouse measured.

g. ELISA of serum TRAP5b levels in 6-month-old male Malat1^+/+, Malat1^−/−, and Malat1^−/−;Malat1^Tg/Tg mice. n = 4-5 mice per group.

h, i. 6-month-old Malat1^+/+, Malat1^−/−, and Malat1^−/−;Malat1^Tg/Tg mice received intratibial injection of 5,000 B16F1 melanoma cells. Bioluminescent imaging of live animals was performed at the indicated times (h) and photo flux was quantitated at day 25 (i).

Statistical significance in a-g and i was determined by a two-tailed unpaired t-test. Error bars are s.e.m.