Figure 4.

Schematic representation of the design of Apt-clean bispecific aptamer molecules. Essentially, Apt-clean bispecific aptamers consist of an aptamer for a POI (e.g., FGFR1) colored in blue, and another aptamer for a protease (e.g., thrombin) colored in green; generally, a linking moiety is not needed in between the aptamers. The degradation pathway is not UPS-dependent; instead, it relies on the protease activity of thrombin to initiate catalytic proteolysis.