Table 1.

Currently disclosed oligonucleotide-based degraders with the E3 ligase used, target proteins, cell lines tested, and core DNA/RNA sequences used. The DNA/RNA sequences may be extended or shortened, or the bases may be chemically modified. More information can be found in the corresponding references.

Names	E3 Ligases	POIs	Cell Lines	Ref.
	Core DNA/RNA Sequences Used
TRAFTAC	VHL	NF-ĸB and brachyury	HeLa	[16]
	To generate double-stranded DNA for recruiting POIs, a NF-ĸB binding sequence 5′-GGGAATTTCC-3′ and a brachyury binding sequence 5′-AATTTCACACCT-3′ were referenced.
OligoTRAFTAC	VHL	cMYC and brachyury	HeLa and HEK293T	[17]
	To generate double-stranded warheads, a cMYC binding consensus sequence 5′-CACGTGGTTGCCACGTG-3′ and a brachyury binding DNA sequence 5′-AATTTCACACCTAGGTGTGAAATT-3′ were referenced.
O’PROTAC	VHL and CRBN	binding factor 1 (LEF1) and ETS-related gene (ERG)	PC-3	[18]
	A 18-mer oligonucleotide, 5′-TACAAAGATCAAAGGGTT-3′, was referenced for generating double-stranded DNA specific for targeting LEF1.A 19-mer oligonucleotide, 5′-ACGGACCGGAAATCCGGTT-3′, was referenced for generating double-stranded DNA specific for targeting ERG.
TF-PROTAC	VHL	E2F and the subunit of NF-ĸB, p65	HeLa	[19]
	A single-stranded DNA sequence of 5′-TGGGGACTTTCCAGTTTCTGGAAAGTCCCCA-3′ was used as a warhead to target the subunit of NF-ĸB, p65, while double-stranded DNA 15-mers (sense chain was 5′-CTAGATTTCCCGCG-3′ and the antisense chain was 5′-CTAGCGCGGAAAT-3′) were selected to bait cancer-related E2F.
SNIPER	IAP	ERα	MCF7	[20]
	The 21-mer, ERα-specific, double-stranded DNA was referenced from 5′-GTCAGGTCACAGTGACCTGAT-3′.
Aptamer-PROTAC conjugates	VHL	BRD2, 3, and 4	MCF7	[21]
	The 26-mer AS1411 was 5′-GGTGGTGGTGGTTGTGGTGGTGGTGG-3′.
Aptamer-warheaded PROTAC	VHL	nucleolin	MCF7 and BT474	[22]
	The same AS1411 sequence as above was used.
Light controllable, aptamer-warheaded PROTAC	CRBN	nucleolin	MCF7 and MDA-MB-231	[23]
	The same AS1411 sequence as above was used.
RNA-warheaded PROTAC	VHL/HIF-1α	LIN28 and RBFOX1	K562	[24]
	5′-AGGAGAU-3′ was for Lin28, while 5′-UGCAUGU-3′ was for RBFOX1.
G4-PROTAC	VHL and CRBN	RHAU	HeLa	[25]
	RHAU-targeting G4 sequence was TT(GGGT)4.
Apt-clean	No E3 used; instead, thrombin protease	FGFR1	3T3-L1	[26]
	The aptamer sequence of HD1 was 5′-GGTTGGTGTGGTTGG-3′, while the aptamer sequence of HD22 was 5′-AGTCCGTGGTAGGGCAGGTTGGGGTGACT-3′.A 38-mer DNA aptamer of FGFR1-binding SL38.2 was 5′-CGATCGATGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAATCGATCG-3′.

The underlines in the DNA sequences represent core binding moieties specific for corresponding protein targets.