Table 4.
EV classification and their biophysical characteristics.
| EV Subpopulation |
Diameter | Sedimentation in Centrifugation | Molecular Markers | Cellular Origin |
|---|---|---|---|---|
| Exosomes | 40–150 nm | 100,000–200,000× g
for 1–2 h |
CD9, CD63, CD81, TSG101, Alix | Multivesicular bodies |
| Microvesicles (or ectosomes) |
100–1000 nm | 10,000–20,000× g
for 10–20 min |
Integrins, selectins, CD40 ligand | Plasma membrane budding |
| Apoptotic bodies (or apoptotic cell-derived EVs) | 1–5 µm | 1000–2000× g
for 10–20 min |
Histones, Annexin V |
Apoptotic cell membrane blebbing |