Table 6.
Mutagenic Activity and Mutagenic Index (Displayed in Parenthesis) of 5a against S. typhimurium TA98 and TA100 Strainsa
| number of revertants/plate and (MI) |
||||
|---|---|---|---|---|
| TA98 |
TA100 |
|||
| concentration (μg/plate) | S9− | S9+ | S9− | S9+ |
| 0b | 25.67c ± 5.51 | 24.33 ± 9.50 | 206.00 ± 7.07 | 201.00 ± 9.90 |
| 50 | 26.00 ± 1.41 (1.01) | 32.00 ± 11.31 (1.31) | 190.50 ± 4.95 (0.92) | 185.00 ± 7.07 (0.92) |
| 150 | 34.50 ± 0.71 (1.34) | 35.00 ± 1.41 (1.43) | 187.50 ± 3.54 (0.91) | 187.00 ± 5.66 (0.93) |
| 500 | 38.00 ± 2.83 (1.48) | 43.50 ± 6.36 (1.78) | 233.00 ± 7.07 (1.13) | 191.50 ± 9.19 (0.95) |
| 1500 | 39.00 ± 9.90 (1.51) | 30.50 ± 3.54 (1.25) | 241.00 ± 2.83* (1.16) | 198.50 ± 4.95 (0.98) |
| 5000 | 37.50 ± 9.19 (1.46) | 43.50 ± 0.71 (1.78) | 241.00 ± 1.41* (1.16) | 227.00 ± 9.90 (1.12) |
| control | 34.50 ± 2.12d | 137.00 ± 7.07f | 260.70 ± 15.30e | 1256.00 ± 102.10f |
*
P < 0.05 (ANOVA) concentratons of 5a compared with negative control.
a
The results displayed represent the data for the assays in the absence (S9−) and presence (S9+) of an exogenous metabolism system.
b
Negative control (DMSO).
c
Number of revertant colonies.
d
4-Nitrophenylenediamine (NPD, 10 μg/plate).
e
Sodium azide (2.5 μg/plate).
f
2-Aminoanthracene (AA, 2.5 μg/plate).
The results are reported as means ± SD.