Impact of non-digested and digested Ag NMs on HCT116 cell viability. Cell viability assessed by WST-1 assay 24 h after HCT116 cells were exposed to digested and non-digested Ag NPs (a–c), as well as HEC (no Ag) (d) and OGI fluid (no NMs) (e). PC refers to the positive control, i.e., amino-modified polystyrene nanoparticles (PSNH2) used at 100 µg/mL. Ag intracellular accumulation (f). HCT116 cells were exposed for 24 h to AgNKD, AgPVP and AgHEC, and intracellular Ag content was measured using ICP-MS. Values are the mean ± SD of three independent experiments with five replicates per experiment. Statistical significance, exposed vs. control, (*) p < 0.05, (***) p < 0.001, (****) p < 0.0001.