Figure 6.

Results from apoptosis assays. A2780 cells were treated with 0.5 µM pc-I, pc-I + UV, I or vehicle (DMSO). (A) Cells were stained with annexin V and propidium iodide (PI) after 48 and 72 h. (B) Annexin-V-positive but PI-negative cells were classified as early apoptotic; annexin-V- and PI-positive cells were classified as late apoptotic. Statistical difference was calculated for annexin-V-positive cells compared to vehicle. (C) Cells were treated with the compounds and vehicle (DMSO) for 24 h, followed by incubation with caspase-Glo 3/7 substrate, and subsequently, luminescence was measured. For primary data, see Supporting Information Figure S2. Statistical analysis was performed with one-way ANOVA (*** p < 0.001).