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. 2023 Mar 10;28(6):2542. doi: 10.3390/molecules28062542

Figure 8.

Figure 8

Effect of inhibitors on biofilm formation by staphylococci. (A) Staphylococcal cells were incubated with 40 μg/well of each inhibitor at 37 °C for 24 h. Biofilm was stained with crystal violet, and the absorbance was measured at 595 nm. (B) Staphylococcal cells were incubated with increasing concentration of ASME at 37 °C for 24 h. Biofilm formation was detected as indicated above. Values are expressed as percentage of the control (no inhibitor added). Data indicate the average of two independent experiments, each performed in quadruplicate. p values were determined using a two-tailed, two-sample unequal variance Student’s t-test in Prism 4.0 (GraphPad, Prism 4.0, San Diego, CA 92121, USA) with *** p < 0.001 and **** p < 0.0001 vs. control.