Figure A7.

Heatmap of species-wise standardized counts illustrating species modules identified by spectral clustering (shown as the two top bars). Columns represent individual species and are ordered by the clusters predicted from the affinity matrix based on the BZINB correlations between species in the disease (ECC) group. Columns are annotated to show and compare the predicted clusters between healthy (no ECC) and disease (ECC) groups. Each row represents a participant (n = 289). The rows are ordered based on hierarchical clustering and are annotated to illustrate healthy and disease groups and the sequencing batch. Standardized counts were calculated separately for each species by first suppressing outliers (defined by greater than 3 × IQR from the first or third quartile), then centering around the median and dividing by the standard deviation.