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. 2023 Feb 27;13(3):281. doi: 10.3390/membranes13030281

Figure 1.

Figure 1

Effect of fixed concentrations of pooled immunoglobulin G (10 mg/mL), hematin (80 µM) and melittin (0.2 µg/mL Mt; 0.5 µg/mL Mt; 2 µg/mL Mt and 5 µg/mL Mt) treatments on hematocrit (average value ± SD) of erythrocytes (a) with an Rhesus-positive factor and (b) Rhesus-negative factor. The pre-incubated sample of pooled immunoglobulin G (10 mg/mL) with hematin (80 µM) is indicated by an asterisk on the abscissa. Erythrocytes were treated with biomacromolecules for 1 h at 37 °C in phosphate buffered saline (PBS), pH 7.4. Hematocrit determination was performed at 25 °C. Each value is the mean ± SD of three independent preparations with 8–15 repetitions each. *** p < 0.001; ** p < 0.01; * p < 0.05 compared to the untreated control (red asterisks) or DMSO (80 µM)—control (green asterisks).