Table 2.

Single-factor optimization: process and formulation.

		Value	Size	PDI	Zeta
L-HA–PDM	DM (μL)	100	445.99 ± 16.94	0.51 ± 0.16	−36.98 ± 2.41
		300	272.53 ± 8.32	0.32 ± 0.04	−31.64 ± 3.09
		500	276.06 ± 10.42	0.29 ± 0.04	−32.08 ± 1.98
	KPS (mL)	0.5	340.11 ± 13.16	0.46 ± 0.06	−37.29 ± 2.19
		1.5	255.68 ± 12.94	0.26 ± 0.05	−31.58 ± 1.97
		2.5	522.62 ± 14.39	0.33 ± 0.13	−23.42 ± 2.05
	Stirring speed (rpm)	1040	577.09 ± 15.92	0.34 ± 0.13	−32.27 ± 1.97
		1300	324.18 ± 11.68	0.22 ± 0.08	−31.64 ± 1.67
		1560	451.67 ± 9.97	0.21 ± 0.07	−34.92 ± 2.21
	Temperature (°C)	50	250.48 ±17.97	0.17 ± 0.07	−42.02 ± 4.02
		60	286.22 ± 18.66	0.15 ± 0.11	−40.68 ± 4.19
		70	445.99 ± 16.94	0.51 ± 0.16	−36.98 ± 2.41
M-HA-PDM	DM (μL)	100	534.02 ±25.64	0. 62 ± 0.18	−33.96 ± 2.89
		300	762.46 ± 19.64	0.54 ± 0.13	−36.39 ± 2.06
		500	453.04 ± 15.62	0.32 ± 0.09	−34.88 ± 2.14
	KPS (mL)	0.5	728.25 ± 17.69	0.33 ± 0.11	−36.92 ± 2.01
		1.5	443.11 ± 15.21	0.28 ± 0.09	−38.57 ± 1.97
		2.5	412.67 ± 12.71	0.23 ± 0.08	−35.04 ± 2.15
	Stirring speed (rpm)	1040	292.07 ± 11.29	0.24 ± 0.05	−33.84 ± 2.67
		1300	264.82 ± 11.06	0.16 ± 0.08	−34.02 ± 2.81
		1560	437.43 ± 10.11	0.21 ± 0.07	−38.59 ± 2.09
	Temperature (°C)	50	452.39 ± 13.42	0.19 ± 0.08	−27.99 ± 1.87
		60	262.59 ± 10.97	0.15 ± 0.04	−36.52 ± 3.14
		70	269.55 ± 10.67	0.16 ± 0.04	−26.98 ± 1.62
H-HA–PDM	DM (μL)	100	452.37 ± 19.64	0.67 ± 0.21	−28.64 ± 2.61
		300	304.62 ±16.35	0.33 ± 0.16	−32.05 ± 2.01
		500	287.88 ± 12.62	0.47 ± 0.11	−28.64 ± 1.99
	KPS (mL)	0.5	557.41 ± 16.98	0.38 ± 0.10	−38.65 ± 2.23
		1.5	400.21 ± 14.62	0.32 ± 0.08	−41.02 ± 1.97
		2.5	239.11 ± 10.98	0.24 ± 0.05	−37.88 ± 2.09
	Stirring speed (rpm)	1040	306.13 ± 15.09	0.22 ± 0.09	−30.94 ± 2.64
		1300	332.82 ± 12.01	0.18 ± 0.05	−26.48 ± 1.96
		1560	277.98 ± 10.93	0.14 ± 0.04	−29.54 ± 1.89
	Temperature (°C)	50	592.59 ± 18.19	0.16 ± 0.07	−34.62 ± 3.01
		60	395.92 ± 10.67	0.15 ± 0.08	−31.82 ± 1.67
		70	275.24 ± 8.00	0.14 ± 0.05	−25.61 ± 1.98