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. 2023 Mar 16;24(6):5705. doi: 10.3390/ijms24065705

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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COA6 regulates the MMP, ROS, and NADH of LUAD cells (A549 and H1975). (A) Fluorescence staining showing the MMP affected by siRNA, Magnification: 200×; (B) Fluorescence staining showing the ROS affected by siRNA, Magnification: 200×; (C,D) The histograms showing that knockdown of COA6 decreased MMP of LUAD cells (mean ± SD, C: n_control = 11, n_coa6 = 13, D: n_control = 17, n_coa6 = 11); (E,F) The histograms showing that knockdown of COA6 did not decrease the ROS in A549 cells significantly, but decreased the ROS in H1975 cells significantly (mean ± SD, C: n_control = 30, n_coa6 = 32, D: n_control = 22, n_coa6 = 13); (G,H) The histograms showing that knockdown of COA6 decreased NADH in LUAD cells (mean ± SD, n = 3). Independent samples t−test was used in comparing the means of two groups, * p < 0.05 and *** p < 0.001.