Figure 6.

Schematic diagram of the generation of a recombinant poxvirus by homologous recombination of parental virus and a transfer vector. The plasmid encoding the transfer vector contains two antigen genes (purple arrows), an enhanced green fluorescent protein gene (egfp) as a marker gene (green arrow) as well as left and right flanks (49 and 50 flanks, respectively; blue arrows). Overall, 49, left flank of the transfer vector, ±300 bp of the 3′ end of 049 ORF of the LSDV genome; 50, right flank of the transfer vector, ±300 bp of the 3′ end of 050 ORF of the LSDV genome. All genes expressed by poxviruses are under the control of poxvirus promoters [99] (From student thesis where I am the superviso).