VitC effect on AP generation. Representative images of Live/Dead staining showing the effect of adding VitC to AMX on H. pylori cells during the AP generation. Monitoring occurred after 48 h, 72 h, and 96 h of incubation, and a comparison with the untreated sample was conducted (AMX). The second treatment with AMX guaranteed the complete killing of the population growing in AMX plus VitC for 48 h. Original magnification, 1000×. In the graphic, the MTT values show an increase in the viability of the H. pylori cells in AMX +VitC for 48 h, compared to those grown only in AMX (untreated sample).