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. 2023 Feb 21;13(3):379. doi: 10.3390/jpm13030379

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Compared with the control group, the MLN4924 group had increased expression levels of ABCG2 and Bmi1. (B) The K12 mRNA levels decreased after MLN4924 treatment. (C) Immunofluorescence staining showed that MLN4924 suppressed the expression of K12. (D) Western blotting showed that the C/EBPδ protein expression level increased in LSCs after MLN4924 treatment, whereas the protein level of K12 decreased. (E) Statistical analysis revealed a higher protein expression level of C/EBPδ and a lower protein level of K12. All of the experiments were independently repeated four times. * p < 0.05 and ** p < 0.01. Scale bar: 100 μm.