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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Mol Cell Endocrinol. 2023 Jan 20;563:111864. doi: 10.1016/j.mce.2023.111864

Figure 3. Differential Chromatin Accessibility in Response to Dex in Embryonic Cortical NSPCs.

Figure 3.

A) Points representing an ATAC-seq differential peaks between vehicle- vs. Dex-treated samples (n=95). Fold change of normalized average ATAC-seq peak read intensity (Dex-Veh) (x-axis); p-value (p<0.05) (y-axis).

B) HOMER motif analyses predict enrichment of GRE and/or SOX TF motifs (x-axis) under (i) all differential ATAC-seq peaks (black), differential ATAC-seq peaks induced by Dex (blue), or differential ATAC-seq peaks attenuated by Dex (pink). HOMER motif predictions were called using a (p<0.05) cut-off for significance.

C) A model of TSS-proximal TF motifs with facilitate the dynamic chromatin response to Dex in embryonic cortical NSPCs. Each vertical bar represents a cluster of ATAC-seq reads, with a set of bars representing a single ATAC-seq peak. The vertical height of each bar represents intensity of ATAC-seq reads at a single genomic location.

D) Peak ID of differential ATAC-seq peaks (p<0.05), which occur within the −5kb/+3kb region of a Dex-regulated gene, or at a Dex-regulated gene TSS. Asterisks indicate ATAC-seq peaks with a Dex-induced decrease in chromatin accessibility. RNA-seq data previously published by Frahm et al., 201810. RNA-seq log Veh/Dex fold change.