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. 2023 Mar 18;15(6):1470. doi: 10.3390/nu15061470

Figure 2.

Figure 2

In vivo effects of chronic kidney disease (CKD) and dietary phosphorus intake on kidney α-Klotho expression and its correlation with sKlotho (A) Kidney α-Klotho mRNA levels from Sham-operated fed normal phosphorus (NP) (Reference) and CKD mice fed NP or high phosphorus (HP) diet for 14 weeks. All values are expressed relative to reference group. Mean and individual mice values are shown. aaa p < 0.005 vs. Reference, b p < 0.05 vs. CKD+NP. R.U., relative units vs. Reference. (B) Representative image of Western blot analysis and relative quantification. All values are expressed relative to the reference group. Horizontal lines depict mean values. aaa p < 0.005 vs. Reference, b p < 0.05 vs. CKD+NP. R.U.: Relative Units vs. reference group. (C) Representative images of kidney α-Klotho and its quantification. Each inset indicates the relative scale in µm. a p < 0.05 vs. Reference, aaa p < 0.005 vs. Reference, b p < 0.05 vs. CKD+NP. (D) Correlation between kidney α-Klotho protein expression and soluble Klotho (sKlotho) levels within CKD groups. Each shape represents, CKD+NP (●) and CKD+HP (■) values. GAPDH was used as housekeeping gene or loading control.