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. 2023 Mar 18;15(6):1470. doi: 10.3390/nu15061470

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vivo effects of chronic kidney disease (CKD) and dietary phosphorus intake on aortic osteogenic differentiation and components of the Wnt/β-catenin pathway. (A) α-actin and (B) Sost mRNA levels in abdominal aortas from Sham-operated (Reference) and CKD mice fed a normal (NP) or high phosphorus (HP) diet for 14 weeks. All values are expressed relative to reference group. Mean and individual mice values are shown. ^a p < 0.05 vs. Reference. R.U., relative units vs. reference group. GAPDH was used as housekeeping gene. (C) Correlation between α-actin mRNA levels and serum sKlotho in all groups. Each shape represents Reference (○), CRF+NP (●) and CRF+HP (■) values.