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. 2023 Mar 18;15(6):1470. doi: 10.3390/nu15061470

Figure 6.

Figure 6

sKlotho effect on primary vascular smooth muscle cells (VSMCs) differentiation to osteoblast-like cells. (A) Ca content quantification, (B) Osterix (C) α-actin and (D) Sost mRNA levels in primary VSMCs exposed to non-calcifying medium (Non-CM), calcifying medium (CM) and CM+sKlotho for 3 days. All values are expressed relative to Non-CM condition as mean of 3 independent experiments by triplicates (horizontal lines). a p < 0.05, aa p < 0.01 and aaa p < 0.005 vs. Non-CM, b p < 0.05 and bb p <0.01 vs. CM. R.U., relative units vs. Non-CM. GAPDH was used as housekeeping gene.