Table 8.

PCR assays used to genotype Solina accessions for alleles at major vernalization and photoperiod loci.

Primer Name	Primer Sequence [5′–3′]	References
VRN1AF VRN1R	GAAAGGAAAAATTCTGCTCG TGCACCTTCCC(C/G)CGCCCCAT	[26]
Intr1/A/F2 Intr1/A/R3	AGCCTCCACGGTTTGAAAGTAA AAGTAAGACAACACGAATGTGAGA	[27]
Intr1/C/F Intr1/AB/R	GCACTCCTAACCCACTAACC TCATCCATCATCAAGGCAAA	[27]
Intr1/B/F Intr1/B/R3	CAAGTGGAACGGTTAGGACA CTCATGCCAAAAATTGAAGATGA	[27]
Intr1/B/F Intr1/B/R4	CAAGTGGAACGGTTAGGACA CAAATGAAAAGGAATGAGAGCA	[27]
VRN4-B-INS-F VRN4-B-INS-R	CATAATGCCAAGCCGGTGAGTAC ATGTCTGCCAATTAGCTAGC	[28]
VRN4-B-NOINS-F VRN4-B-NOINS-R	ATGCTTTCGCTTGCCATCC CTATCCCTACCGGCCATTAG	[28]
TaPpd-A1prodelF TaPpd-A1prodelR3	CGTACTCCCTCCGTTTCTTT AATTTACGGGGACCAAATACC	[19]
TaPpd-A1prodelF TaPpd-A1prodelR2	CGTACTCCCTCCGTTTCTTT GTTGGGGTCGTTTGGTGGTG	[19]
TaPpd-B1proinF1 TaPpd-B1proinR1	CAGCTCCTCCGTTTGCTTCC CAGAGGAGTAGTCCGCGTGT	[19]
Ppd-D1_F1 Ppd-D1_R2	ACGCCTCCCACTACACTG CACTGGTGGTAGCTGAGATT	[29]
Ppd-D1_F1 Ppd-D1_R1	ACGCCTCCCACTACACTG GTTGGTTCAAACAGAGAGC	[29]