Table 10.
Amplification conditions used in digital-PCR.
| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Activation | 96 °C | 10 min | 1 |
| Denaturation | 98 °C | 30 s | 47 |
| Annealing/extension | 56 °C | 2 min | |
| Final extension | 60 °C | 2 min | 1 |
| Storage | 4 °C | ∞ | 1 |
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