. 2023 Feb 27;89(3):e02123-22. doi: 10.1128/aem.02123-22

TABLE 2.

Primers used in the study

Primer	Sequence (5′–3′)	Purpose
ineAM-F	CGCCATATGATGGAAAAACTGAACCATAACG	Amplifying the ineAM fragment
ineAM-R	CGCGGATCCTTAGAAAAAGCTCAGATTCAG	Amplifying the ineAM fragment
ineT₁₅₀-F	GGAATTCCATATGTTGAAACTAGTATTACAAAATAATG	Amplifying the ineT₁₅₀ fragment
ineT₁₅₀-R	ACGCGTCGACTTATTTTTTAATTAAATCAACCAAAG	Amplifying the ineT₁₅₀ fragment
PineA-F	CGCGGATCCATGGAAAAACTGAACCATAACG	Amplifying the ineA leader peptide sequence
PineA-R	CACCAACGATTGCCGCCCAGGATGGTATC	Amplifying the ineA leader peptide sequence
MmutA-F	CATCCTGGGCGGCAATCGTTGGTGGCAAGGTG	Amplifying the mutA core peptide sequence
MmutA-R	ACTTCAACTTCTTAACAGCAAGTGAAAACATG	Amplifying the mutA core peptide sequence
H13Y-L1	CGACAGTTAGCTATGAATGTCGTATGAACTCCTTTC	Constructing the inecin L (H13Y) expression vector
H13Y-S2	GCAGCACGCCCTGCCACCAAC
H13Y-L2	TCATACGACATTCATAGCTAACTGTCGGCAGCACG
H13Y-S1	ACTCCTTTCAGCATATCTTTAC
Y13H-L1	ACGGTCTCACATGAGTGTCGCATGAATTCATGGC	Constructing the mutacin II (Y13H) expression vector
Y13H-S2	TGGCACAACACCTTGCCACCAACG
Y13H-L2	GACACTCATGTGAGACCGTTGGCACAACACCTTGCC
Y13H-S1	GCATGAATTCATGGCAACATG
S1N-L1	TGGGCGGCAACCGTTGGTGGCAGGGCGTGCTGCCGACAG	Constructing the inecin L (S1N) expression vector
S1N-S2	GGATGGTATCCAGTTCGCTATCG
S1N-L2	ACCAACGGTTGCCGCCCAGGATGGTATCCAGTTCGCTATC
S1N-S1	GGCAGGGCGTGCTGCCGACAGTTAG
L8V-L1	AGGGCGTGGTGCCGACAGTTAGCCATGAATGTCGTATG	Constructing the inecin L (L8V) expression vector
L8V-S2	GCCACCAACGTGAGCCGCCCAGGATG
L8V-L2	ACTGTCGGCACCACGCCCTGCCACCAACGTGAGCCGCCCAG
L8V-S1	TAGCCATGAATGTCGTATGAACTC
F20W-L1	ATGAACTCCTGGCAGCATATCTTTACCTGCTGTTAAGAAG	Constructing the inecin L (F20W) expression vector
F20W-S2	ACGACATTCATGGCTAACTGTCG
F20W-L2	ATATGCTGCCAGGAGTTCATACGACATTCATGGCTAACTG
F20W-S1	CTTTACCTGCTGTTAAGAAGTTG
I23V-L1	TTCAGCATGTGTTTACCTGCTGTTAAGAAGTTGAAGTAAAG	Constructing the inecin L (I23V) expression vector
I23V-S2	AGGAGTTCATACGACATTCATGG
I23V-L2	AGGTAAACACATGCTGAAAGGAGTTCATACGACATTCATG
I23V-S1	GCTGTTAAGAAGTTGAAGTAAAG