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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Adv Mater. 2023 Jan 11;35(10):e2208781. doi: 10.1002/adma.202208781

Figure 3. MSCM-coating rescued MSC osteogenesis and mineral deposition in vitro by inducing CD8+ T cell apoptosis while enhancing Treg differentiation.

Figure 3.

(a-c) Evaluating the effects of MSCM coating on TCD8+ apoptosis using flow cytometry; (b) representative flow cytometry plots and (c) quantification of apoptotic CD8+ cells, (d-e) Evaluating the effects of conditioned medium (CM) from TCD8+ cultured under different conditions on MSC mineralization using ARS staining; (f-h) Evaluating the effects of MSCM coating on Treg differentiation; (g) Representative flow cytometry plots and (h) quantification of Tregs (of CD25+ FoxP3+); (i-j) Evaluating the effects of CM from Treg cultured under different conditions on MSC mineralization; Data are represented as mean ± S.D. (n = 4/group). Scale bars: 200 μm. **P < 0.01, # indicates P < 0.001 when compared with all other groups.