Table 1.
Top MS/MS-identified metabolites, exhibiting the greatest degree of change in all tissues when comparing basal versus higher-fat diets.
| Tissue | Metabolite Name | log2(Fold Change) | −log10(p-Value) | Frequency: Basal (n = 5) |
Frequency: Higher-Fat (n = 5) |
|---|---|---|---|---|---|
| Brain Cortex | S-adenosylhomocysteine | 18.99 | 1.77 | 0 | 3 |
| Brain Cortex | Glutathione | −21.95 | 1.90 | 4 | 0 |
| Heart | Arabitol | −17.62 | 2.12 | 3 | 0 |
| Heart | Betaine | −17.19 | 1.45 | 4 | 0 |
| Kidney | 1-(Octadecenoyl)-sn-glycero-3-phosphoethanolamine | 15.31 | 2.27 | 0 | 3 |
| Kidney | Indoxyl sulfate | −17.57 | 1.35 | 3 | 0 |
| Liver | 1-(Octadecenoyl)-sn-glycero-3-phosphoethanolamine | 14.88 | 2.06 | 0 | 4 |
| Liver | Glycocholic acid | 4.73 | 1.66 | 1 | 5 |
| Liver | 1-O-Hexadecyl-sn-glycero-3-phosphocholine | −17.12 | 1.92 | 4 | 0 |
| Skeletal Muscle | Inosine | 19.55 | 1.64 | 0 | 4 |
| Skeletal Muscle | Uridine diphosphate glucuronic acid | 15.00 | 1.65 | 0 | 4 |
| Skeletal Muscle | 7α-Hydroxy-3-oxo-4-cholestenoic acid | 1.85 | 1.81 | 4 | 5 |
| Skeletal Muscle | 1-(Octadecenoyl)-sn-glycero-3-phosphoethanolamine | 1.83 | 1.46 | 1 | 5 |
| Pancreas | 1-(Octadecenoyl)-sn-glycero-3-phosphoethanolamine | 2.38 | 1.42 | 1 | 5 |
| Pancreas | Trimethylamine N-oxide | −2.64 | 1.54 | 5 | 4 |
| Pancreas | Betaine | −2.45 | 1.85 | 5 | 3 |
Criteria for Selection: Frequency ≥ 60% in any one cohort; −log10(p-value) ≥ 1.3; |log2(Fold-Change)| ≥ 1.5; Median abundance values ≥ 25,000. Note: a positive fold-change when comparing a basal diet versus a higher-fat diet denotes an increase in abundance for the higher-fat cohort. n equals the number of samples in the cohort in which that metabolite was identified. Features were putatively identified via MS/MS.