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. 2023 Mar 16;24(6):5717. doi: 10.3390/ijms24065717

Figure 3.

Figure 3

Validation of double and triple combinations in the paediatric AML cell lines. (A) Flow cytometry analysis of the annexin V-positive cell population for Kasumi-1 and MV4-11 cells following 72 h treatment as single agents and as a combination. (B) Western blot analysis of PARP, cleaved-PARP, procaspase-9, cleaved caspase-9, procaspase-3, and cleaved caspase-3 in Kasumi-1 and MV4-11 cell lines following 72 h treatment as single agents and as a combination. GAPDH was used as a loading control. All results shown are representative of three independent experiments. (C) Reactive oxygen species quantification following 72 h treatment as single agents and as a combination. Stained cells were analysed for fluorescence, measured using a Synergy HTX Multi-Mode Micro-Plate reader. We used 5 mM H2O2 and 5 mM N-acetyl cysteine (NAC) anti-oxidant as positive and negative controls respectively. * = p < 0.05, ** = p < 0.005 values of significance of treatments compared to controls were calculated using the paired t-test.