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. 2023 Mar 17;15(3):777. doi: 10.3390/v15030777

Figure 2.

Figure 2

PRRSV viral load of different infection groups. PAMs in the PCV2 alone group (PCV2), the PRRSV alone group (PRRSV) and the PCV2 and PRRSV co−infected group (PCV2−PRRSV, PRRSV−PCV2 and PCV2 + PRRSV) were inoculated with PRRSV and/or PCV2 at a MOI of 1, respectively. PAMs in the mock group were inoculated with equal RPMI−1640 medium. Then, PAMs from these groups were collected at 6 h, 12 h, 24 h, 36 h and 48 h post−infection, respectively. (A) The levels of viral RNA were measured by RT−qPCR. (B) The viral titer of PRRSV in the supernatants was analyzed by TCID50. Data are expressed as means ± SD of three independent experiments. Statistically significant differences for the PCV2 and PRRSV co−infected groups vs. the mock−inoculated group and the PCV2 alone group are indicated (*** p < 0.001). Significant differences between groups are also represented (** p < 0.01, * p < 0.05).