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. 2023 Mar 17;15(3):777. doi: 10.3390/v15030777

Figure 7.

Figure 7

Relative mRNA expression of immune checkpoints. PAMs in the PCV2 alone group (PCV2), the PRRSV alone group (PRRSV) and the PCV2 and PRRSV co-infected groups (PCV2–PRRSV, PRRSV–PCV2 and PCV2 + PRRSV) were inoculated with PRRSV and/or PCV2 at a MOI of 1, respectively. PAMs in the mock group were inoculated with equal RPMI-1640 medium. Then, PAMs from these groups were collected at 6 h, 12 h, 24 h, 36 h and 48 h post-infection, respectively. RT-qPCR analysis of PD-1 (A), CTLA4 (B), LAG3 (C) and TIM3 (D) mRNAs in PAMs from different groups. Data are expressed as means ± SD of three independent experiments. Significant differences between groups are represented (** p < 0.01, * p < 0.05).