Table 2.
Primer sequences of the reference genes.
| Genes | Primer Sequence (5’–3’) |
|---|---|
| PCV2 | F 5’-CCTCACCTATGACCCCTAT-3’ |
| R 5’TGTTGTTTGGTTGGAAGTA-3’ | |
| PRRSV | F 5’-AAACCAGTCCAGAGGCAAGG-3’ |
| R 5’GCAAACTAAACTCCACAGTGTAA-3’ | |
| IFN-α | F 5’-GGATCAGCAGCTCAGGG-3’ |
| R 5’GAGGGTGAGTCTGTGGAAGTA-3’ | |
| IFN-γ | F 5’-AAAGATAACCAGCCCATTC-3’ |
| R 5’-GTCATTCAGTTTCCCAGA-3’ | |
| TNF-α | F 5’-TGGTGGTGCCGACAGATGG-3’ |
| R 5’-GGCTGATGGTGTGAGTGAGGAA-3’ | |
| IL-1β | F 5’-ATGCTGAAGGCTCTCCACCTC-3’ |
| R 5’-TTGTTGCTATCATCTCCTTGCAC-3’ | |
| IL-10 | F 5’-GCATCCACTTCCCAACCA-3’ |
| R 5’GCAACAAGTCGCCCATCT-3’ | |
| TGF-β | F 5’-CTTACTGAGCATCTTGGACCTTA-3’ |
| R 5’CCACTGAGCCACAATGGAAA-3’ | |
| PD-1 | F 5’-AGCCCAAGCACTTCATCCTC-3’ |
| R 5’-TGTGGAAGTCTCGTCCGTTG-3’ | |
| LAG-3 | F 5’-CTCCTCCTGCTCCTTTTGGTT-3’ |
| R 5’-CAGCTCCCCAGTCTTGCTCT-3’ | |
| CTLA-4 | F 5’-TCTTCATCCCTGTCTTCTCCAAA-3’ |
| R 5’-GCAGACCCATACTCACACACAAA-3’ | |
| TIM-3 | F 5’-TTCGACGGGAGCAGTAAAGC-3’ |
| R 5’-AGGGCAGGACACAGTCAAAG-3’ | |
| β-actin | F 5’-CGGGACATCAAGGAGAAGC-3’ |
| R 5’-CTCGTTGCCGATGGTGATG-3’ |