Fig 1. Identification and morphological characterization of exosomes.

Exosome isolation was confirmed via immunoblotting using the Exo-Check Antibody Array, a transmission electron microscope, and nanoparticle tracking analysis. (A) The exosome compartments of the samples were placed in the pre-printed spots of eight antibodies against known exosome markers (FLOT1, ICAM, ALIX, CD81, CD63, EpCAM, TSG101, and ANXA5), in the positive control, and in the blank (negative control) to examine the presence of exosome-specific proteins. (B) Representative images from transmission electron microscopy showing typical exosome cup-shaped morphology. (C) The size distribution (black line) and cumulative distribution (gray line) of the exosomes were measured using the nanoparticle tracking analysis.