Fig 2. Effect of intestinal epithelial cell-derived exosomes on NF-κB reporter activation in THP-1 monocytic cells.

The supernatants of the normoxia and hypoxia groups were harvested, and the exosome compartment was extracted. THP-1 Blue NF-κB cells were identically stimulated by the obtained exosomes. The black bar indicates the exosomes obtained from the normoxia group, the red bar indicates the exosomes obtained from the hypoxia group, and the gray bar indicates exosomes obtained from the negative control using 10% (v/v) sterile PBS. Results are expressed as mean ± standard deviation of three experiments. NF-κB, nuclear factor-kappa B.