Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sequences of the primers used in RT-qPCR assay.
| Target Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
| β-actin | TGTCACCAACTGGGACGATA | GGGGTGTTGAAGGTCTCAAA |
| AFP | GCCATCGAAATGCCAGGACA | CGCGTGTAGCCAATGAGGAA |
| ALB | GGAAGAGTGGGCACCAAGTG | CACAGACGGTTCAGGATGGC |
| CK18 | ACTCTTGGAGCTGAGACGCA | GAGTTGCTCCATCTGCACCC |
