Table 4.
Bunodosoma caissarum’s new toxins identified through similarity with other described toxins in transcriptome and confirmed in proteome and/or reduced and alkylated peptidome. * These toxin names were suggested according to Oliveira et al. (2006) proposed nomenclature for sea anemone toxins [58].
| Toxin | Trinity Code | Transcript (Identity) |
E-Value | Toxin Family | Tentacle/Mucus | Omic Approach |
|---|---|---|---|---|---|---|
| * U-actitoxin-Bcs3d | TRINITY_DN72667 | U-actitoxin-Bgr3d (42.3%) | 4.4E−13 | Sea anemone type 3 (BDS) potassium channel toxin | −/+ | Proteome, RA peptidome |
| * U-actitoxin-Bcs3a | TRINITY_DN10015 TRINITY_DN5917 TRINITY_DN38124 |
U-actitoxin-Bgr3a (38.1%) | 4.8E−06 | Sea anemone type 3 (BDS) potassium channel toxin | +/+ | Proteome, RA peptidome |
| * U-actitoxin-Bcs2c | TRINITY_DN42790 | U-actitoxin-Ael2c (45.7%) | 1.8E−09 | Sea anemone type 3 (BDS) potassium channel toxin | +/+ | Proteome |
| * Κ-actitoxin-Bcs4m | TRINITY_DN4181 | Kappa-actitoxin-Avd4m (50%) | 1.4E−16 | Sea anemone type 3 (BDS) potassium channel toxin | +/+ | Proteome, RA peptidome |
| U-actitoxin-Bcs2a | TRINITY_DN14686 | U-actitoxin-Bcs2a (100%) | 1.3E−30 | Sea anemone type 3 (BDS) potassium channel toxin | +/+ | Proteome, RA peptidome |
| Bcs Tx3 | TRINITY_DN1024 | type III potassium channel toxin protein, partial (96%) | 1.4E−47 | Sea anemone type 3 (BDS) potassium channel toxin | +/+ | Proteome, RA peptidome |
| * Κ-actitoxin-Bcs2a | TRINITY_DN58216 | Kappa-actitoxin-Ael2a (50%) | 1.2E−08 | Sea anemone type 3 (BDS) potassium channel toxin | −/+ | RA peptidome |
| * ΚΠ-actitoxin-Bcs3e | TRINITY_DN13254 | kappaPI-actitoxin-Avd3e-like (73.5%) |
4.9E−90 | Venom Kunitz type. Sea anemone type 2 potassium channel toxin. | +/+ | Proteome |
| * Π-actitoxin-Bcs2e | TRINITY_DN217 | PI-stichotoxin-Hcr2e (56.4%) | 2.5E−28 | Venom Kunitz type. Sea anemone type 2 potassium channel toxin. | +/− | Proteome |
| * U-actitoxin-Bcs3n | TRINITY_DN6572 | U-actitoxin-Avd3n (74.7%) | 1.6E−51 | Venom Kunitz type. Sea anemone type 2 potassium channel toxin. | +/+ | Proteome, RA peptidome |
| * Π-actitoxin-Bcs3a | TRINITY_DN10770 | PI-actitoxin-Aeq3a-like (85.9%) | 1.2E−59 | Venom Kunitz type. Sea anemone type 2 potassium channel toxin. | −/+ | Proteome |
| * U-actitoxin-Bcs12a | TRINITY_DN7989 | U-actitoxin-Avd12a (74.4%) | 9.4E−38 | EGF-domain peptide | +/+ | Proteome |
| Turripeptide Pal 9.2-like | TRINITY_DN4295 | Turripeptide Pal 9.2 (38.2%) | 1.2E−12 | Kazal-type serine protease inhibitor domain | +/+ | Proteome, RA peptidome |
| * Π-actitoxin-Bcs5a | TRINITY_DN713 | PI-actitoxin-Avd5a (67.4%) | 8E−24 | Kazal-type serine protease inhibitor domain | +/+ | Proteome |
| Basic phospholipase A2 pseudexin A chain-like | TRINITY_DN24551 | Basic phospholipase A2 pseudexin A chain-like (72.8%) | 7.8E−108 | Phospholipase A2 | −/+ | Proteome |
| * Κ-actitoxin-Bcs1a | TRINITY_DN3227 | Kappa-actitoxin-Bgr1a (89.2%) | 1.4E−20 | Sea anemone type 1 potassium channel toxin | −/+ | Proteome |
| * Κ-actitoxin-Bcs3a | TRINITY_DN4127 | Kappa-actitoxin-Aer3a (55.4%) | 1.3E−28 | Sea anemone type 1 potassium channel toxin | +/+ | Proteome |
| * U-actitoxin-Bcs8a | TRINITY_DN1939 | U-actitoxin-Avd8a-like (82.3%) | 1.9E−51 | Sea anemone 8 toxin | +/+ | Proteome |
| * Δ-actitoxin-Bcs2a | TRINITY_DN24411 | Delta-stichotoxin-Sgt2a (48.2%) | 3.1E−15 | Anemone neurotoxin | −/+ | Proteome, RA peptidome |
| Zinc-metalloproteinase nas-13-like | TRINITY_DN24501 | Zinc-metalloproteinase nas-13-like (84.9%) | 3.5E−80 | Astacin | −/+ | Proteome |
| Zinc metalloproteinase nas-4-like | TRINITY_DN2272 | Zinc metalloprotease nas-4-like isoform X2 (82.9%) | 4E−269 | Astacin | +/+ | Proteome |
| Zinc metalloproteinase nas-15-like | TRINITY_DN1535 | Zinc-metalloproteinase nas-15-like (68.2%) | 1.2E−221 | Astacin | +/+ | Proteome |
| Cystatin | TRINITY_DN6968 | Cystatin-like (57.2%) | 7.3E−65 | Cystatin domain | +/+ | Proteome |
RA peptidome = Reduced and alkylated peptidome; NRNA peptidome = Non-reduced and non-alkylated peptidome. Bgr: Bunodosoma granuliferum; Ae: Actinia equina; Av:Anemonia viridis; Bc: Bunodosoma caissarum; Hc: Heteractis crispa; Sg: Stichodactyla gigantea.