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. 2023 Feb 23;15(3):612. doi: 10.3390/v15030612

Figure 2.

Figure 2

Detection of positive-strand PUUV RNA in various tissues of BF bank voles by RNAscope. (A–F): female, RNA-positive, antibody-positive; (A) lung: positive-strand RNA in bronchiolar epithelial cells (yellow arrow); (B) lung: positive signals in interstitial cells with spindle-shaped (black arrow) and round-oval nuclei (yellow arrow) as well as fine, strand-like signals in the interstitium and in the alveolar walls (yellow arrowheads); (C) Glandula submandibularis: positive acini (yellow arrow); (D) heart: positive signals in cardiomyocytes (yellow arrow); (E) cerebrum: positive glia cells (yellow arrows), endothelial cells (yellow arrowhead) and neurons (black arrow); (F) cerebellum: positive-strand RNA in glia cells (yellow arrowheads), Purkinje cells (black arrows) and granule cells (yellow arrow). Total magnification: (A,B,D,F): 400×, (C):200×, (E):100×. Results of the RNAscope analysis of lung, glandula submandibularis, heart, cerebrum and cerebellum of the negative control vole are shown in Figure S6.