Table 1.
Detection of Puumala orthohantavirus (PUUV) RNA by RT-PCR, anti-PUUV IgG antibodies by ELISA and immunohistochemistry (IHC)-based detection of nucleocapsid (N)-protein in bank voles from the district Osnabrück (OS) and the breeding facility (BF).
| No. of Positive/Total Number of Investigated Voles | |||
|
RT-PCR Pos
ELISA Pos (Persistent Infection) |
RT-PCR Pos
ELISA Neg (Acute Infection) |
||
| OS | BF | OS | |
| 36/189 | 12/13 | 3/189 | |
| Detection of N-Protein by IHC | |||
| Total | 19/29 (66%) 10/19 M, 9/19 F |
9/12 (75%) 9/9 F |
3/3 (100%) 2/3 M, 1/3 F |
| Cerebrum | 7/21 (33%) | 2/12 (17%) | 0/2 (0%) |
| (a) Neuron | 2/7 (29%) | 2/2 (100%) | n.a. |
| (b) Glia cells | 4/7 (57%) | 2/2 (100%) | n.a. |
| (c) Endothelial cells | 3/7 (43%) | 0/2 (0%) | n.a. |
| Cerebellum | 1/19 (5%) | 2/12 (17%) | 0/2 (0%) |
| (a) Stratum moleculare | 0/1 (0%) | 2/2 (100%) | n.a. |
| (b) Stratum ganglionare | 0/1 (0%) | 2/2 (100%) | n.a. |
| (c) Stratum granulosum | 1/1 (100%) | 2/2 (100%) | n.a. |
| Lung | 1/28 (4%) | 2/12 (17%) | 1/3 (33%) |
| (a) Bronchiolar epithelial cells | 1/1 (100%) | 2/2 (100%) | 0/1 (0%) |
| (b) Pneumocytes type I | 0/1 (0%) | 0/2 (0%) | 1/1 (100%) |
| (c) IC with spindle-shaped and round-oval nuclei | 1/1 (100%) | 2/2 (100%) | 1/1 (100%) |
| Glandula mandibularis (acini, IC with spindle-shaped nuclei) |
1/16 (6%) | 0/10 (0%) | 0/1 (0%) |
| Glandula parotidea (acini) | 0/16 (0%) | 2/9 (22%) | 0/1 (0%) |
| Tongue (IC with lancet to spindle-shaped nuclei) | 2/14 (14%) | 0/12 (0%) | 1/1 (100%) |
| Liver | 3/26 (12%) | 0/12 (0%) | 2/3 (67%) |
| (a) Kupffer cells | 3/3 (100%) | n.a. | 2/2 (100%) |
| (b) Cells with spindle-shaped nuclei at sinusoid periphery | 0/3 (0%) | n.a. | 1/2 (50%) |
| Pancreas | 1/12 | 3/8 | 0/2 (0%) |
| (a) Acini | 0/1 (0%) | 3/3 (100%) | n.a. |
| (b) Islet cells of Langerhans | 1/1 (100%) | 1/3 (33%) | n.a. |
| (c) IC with spindle-shaped and round-oval nuclei | 1/1 (100%) | 2/3 (67%) | n.a. |
| Stomach/Pars non-glandularis (epithelial cells) | 1/17 (6%) | 2/12 (17%) | 0/1 (0%) |
| Stomach/Pars glandularis (IC with lancet to spindle-shaped nuclei) |
5/13 (38%) | 8/11 (73%) | n.i. |
| Kidney (glomerulum cells *) | 11/28 (39%) | 4/12 (33%) | 3/3 (100%) |
| Testis (sperm precursor cell) | 1/8 (13%) | n.a. | 0/1 (0%) |
| Heart (IC with lancet-shaped nuclei) | 1/28 (4%) | 0/11 (0%) | 0/3 (0%) |
No PUUV N protein was detected in the negative control bank vole. IC, interstitial cells; n.a., not applicable; n.i., not investigated; M, males; F, females; *, glomerulum cells include endothelial cells, mesangial cells and podocytes.